Feb 28, 2013 Analysis of Ku70−/− pancreatic β-cells revealed an accumulation of DNA damage and activation of p53-dependent cellular senescence similar
Ku70 and Ku80 make up the Ku70/80 heterodimer. They are encoded by the Xrcc5 and Xrcc6 genes, respectively, and are highly abundant in both prokaryotes and eukaryotes. The stability of these proteins depends on each other.
Ku70 is a novel Mcl-1 deubiquitinase that could be a potential target for cancer therapy by manipulating Mcl-1 deubiquitination. loss of expression correlates with decreased survival in gall bladder malignancies patients SMAR1-mediated regulation of repair and apoptosis via a complex crosstalk involving Ku70, HDAC6 and Bax. Single-stranded DNA-dependent ATP-dependent helicase. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair. When associated with KU80, binds to double-stranded telomeric and non-telomeric DNA sequences, but not to single-stranded DNA. Recognition of DNA double‐strand breaks during non‐homologous end joining is carried out by the Ku70–Ku80 protein, a 150 kDa heterodimer that recruits the DNA repair kinase DNA‐dependent protein kinase catalytic subunit (DNA‐PKcs) to the lesion. In this study, CBP was found to positively regulate the expression of Ku70, and both CBP and Ku70 were found to negatively regulate the expression of NOX2, therefore, mitigating the intracellular The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins.
The crystal structure of the human Ku heterodimer was determined both alone and bound to a 55-nucleotide DNA element at 2.7 and 2.5 A resolution, respectively. ku70 tert : gl1; ku70; tert: images; None available : phenotypes ; Surovtseva YV, Shakirov EV, Vespa L, Osbun N, Song X, Shippen DE(2007) In contrast to the ku70 mutant, which undergo telomerase-dependent expansion to more than twice the normal length in a single generation, the ku70 tert double mutant displays accelerated telomere shortening and a precocious onset of genome stability. 2021-02-26 · Depletion of CBP and/or Ku70 inhibited cell growth and caused cell death. To understand the roles of CBP and Ku70 in human melanoma cells, we designed and synthesized a set of CBP siRNA and Ku70 Although the ku70 deletion strain was noted to be more sensitive to UV rays than the corresponding wild-type strain, no lethality, severe growth retardation or loss of gene copy numbers could be detected during repetitive rounds of cultivation and induction of heterologous protein production.
Ku70 is an evolutionarily conserved protein that has functions in DNA repair and maintenance [96].
2021-02-26 · Depletion of CBP and/or Ku70 inhibited cell growth and caused cell death. To understand the roles of CBP and Ku70 in human melanoma cells, we designed and synthesized a set of CBP siRNA and Ku70
Eukaryotic Ku is a heterodimer of two polypeptides, Ku70 (XRCC6) and Ku80 (XRCC5), so named because the molecular weight of the human Ku proteins is around 70 kDa and 80 kDa. The two Ku subunits form a basket-shaped structure that threads onto the DNA end. Heterodimers of the 70 and 80 kDa Ku autoantigens (Ku70 and Ku80) activate the DNA‐dependent protein kinase (DNA‐PK).
Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. It is also required for V(D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system.
Ku70 Antibody (MA5-32645) in WB Western blot was performed using Anti-Ku70 Recombinant Rabbit Monoclonal Antibody (JM61-31) (Product # MA5-32645) and a 70kDa band corresponding to Ku70 was observed across cell lines tested. Ku70 Antibody (PA5-27538) in WB Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), Jurkat (Lane 3), HEK293 (Lane 4) and HepG2 (Lane 5). Ku70 is a novel Mcl-1 deubiquitinase that could be a potential target for cancer therapy by manipulating Mcl-1 deubiquitination. loss of expression correlates with decreased survival in gall bladder malignancies patients SMAR1-mediated regulation of repair and apoptosis via a complex crosstalk involving Ku70, HDAC6 and Bax. Single-stranded DNA-dependent ATP-dependent helicase.
Ku70 Antibody (MA5-32645) in WB Western blot was performed using Anti-Ku70 Recombinant Rabbit Monoclonal Antibody (JM61-31) (Product # MA5-32645) and a 70kDa band corresponding to Ku70 was observed across cell lines tested. Ku70 Antibody (PA5-27538) in WB Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), Jurkat (Lane 3), HEK293 (Lane 4) and HepG2 (Lane 5). Ku70 is a novel Mcl-1 deubiquitinase that could be a potential target for cancer therapy by manipulating Mcl-1 deubiquitination. loss of expression correlates with decreased survival in gall bladder malignancies patients SMAR1-mediated regulation of repair and apoptosis via a complex crosstalk involving Ku70, HDAC6 and Bax.
Single-stranded DNA-dependent ATP-dependent helicase.
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The Ku70 NLS contains five lysines (K539, K542, K544, K553 and K556) that were identified as targets for acetylation in vivo. 18, 29 Lysine acetylation in the cytoplasm drives and coordinates key events such as cytoskeleton dynamics, intracellular trafficking, vesicle fusion, metabolism and stress response. 43, 44 We hypothesized that acetylation of the five lysine residues in the Ku70 NLS 2001-03-01 · Ku70:Ku80 complex Source: GO_Central "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium." Gaudet P. , Livstone M.S. , Lewis S.E. , Thomas P.D. Brief Bioinform 12:449-462(2011) [ PubMed ] [ Europe PMC ] [ Abstract ] Anti-Ku70 Antibody (E-5) is a mouse monoclonal IgG 1 κ Ku70 antibody, cited in 60 publications, provided at 200 µg/ml; raised against amino acids 302-609 mapping at the C-terminus of Ku70 of the Ku protein of human origin We present evidence that inactivation of the Ku70 gene leads to a propensity for malignant transformation both in vitro and in vivo. In vitro, Ku70−/− mouse fibroblasts displayed an increased rate of sister chromatid exchange and a high frequency of spontaneous neoplastic transformation.
Cited in 11 reference(s).
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Ku70 Antibody (PA5-27538) in WB Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), Jurkat (Lane 3), HEK293 (Lane 4) and HepG2 (Lane 5).
This antibody reacts with human, rat samples. Cat.No. 10723-1-AP. KD/KO Validated. KU70.
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Heterodimers of the 70 and 80 kDa Ku autoantigens (Ku70 and Ku80) activate the DNA‐dependent protein kinase (DNA‐PK). Mutations in any of the three subunits of this protein kinase (Ku70, Ku80 and DNA‐PKcs) lead to sensitivity to ionizing radiation (IR) and to DNA double‐strand breaks, and V (D)J recombination product formation defects. Ku70 Antibody (MA5-32645) in WB Western blot was performed using Anti-Ku70 Recombinant Rabbit Monoclonal Antibody (JM61-31) (Product # MA5-32645) and a 70kDa band corresponding to Ku70 was observed across cell lines tested. Ku70 Antibody (PA5-27538) in WB Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), Jurkat (Lane 3), HEK293 (Lane 4) and HepG2 (Lane 5). Ku70 is a novel Mcl-1 deubiquitinase that could be a potential target for cancer therapy by manipulating Mcl-1 deubiquitination.
Tested in Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)) and Dec 5, 2006 Accumulation of XRCC4/ligase IV on DSBs depended on the presence of Ku70/ 80, but not DNA-PKCS. We detected a direct interaction between Feb 27, 2014 The KU70-deficient mutant generated herein was effective in improving gene deletion frequency and allowed shorter homology sequences to Monoclonal Antibody for studying Ku70 in the Cell Cycle / Checkpoint research area.